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celltrace (yellow cell proliferation kit)  (Thermo Fisher)


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    Structured Review

    Thermo Fisher celltrace (yellow cell proliferation kit)
    Celltrace (Yellow Cell Proliferation Kit), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/celltrace+(yellow+cell+proliferation+kit)/celltrace+yellow+cell+proliferation+kit/pm39764850-240-8-12
    Average 90 stars, based on 1 article reviews
    celltrace (yellow cell proliferation kit) - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:

    Article Title: NF-κB-regulated VentX expression mediates tumoricidal effects of chemotherapeutics at noncytotoxic concentrations
    Article Snippet: CellTrace yellow cell proliferation kit , Thermo Fisher Scientific , Cat# C34567.

    Article Title: PTPRZ1-targeting RNA CAR-T cells exert antigen-specific and bystander antitumor activity in glioblastoma
    Article Snippet: Ge518_PTPRZ1-KO cells were stained using the CellTraceTM Yellow Cell Proliferation Kit (Invitrogen, C34573), and Ge518_PTPRZ1-KI cells were stained using the CellTraceTM Far Red Cell Proliferation Kit (Invitrogen, C34564).

    Article Title: A methylcellulose/agarose hydrogel as an innovative scaffold for tissue engineering
    Article Snippet: CellTraceTM Yellow Cell Proliferation Kit (Cat. No. C34573; Thermo Fisher Scientic, Waltham, MA, USA) was used to assess the morphology of hBM-MCSs cultured in MC/AGR hydrogel blends.

    Isolation:

    Article Title: Helper T cell immunity in humans with inherited CD4 deficiency
    Article Snippet: Allogenic Mo were isolated from healthy donor whole blood using EasySep Direct Human PBMC Isolation Kit (#19654; Stemcell Technologies) and Human Pan Monocyte cell isolation kit (#130-096-537; Miltenyi Biotech) in accordance with the manufacturer’s instructions. .. Isolated Mo were incubated in the absence or presence of 5 μg/condition of purified antibodies directed against HLA-ABC (clone G46-2.6, #555551; BD Pharmingen) or HLA-DR, DP, and DQ (clone Tü39, #555557; BD Pharmingen) at 37°C under an atmosphere containing 5% CO 2 for 1 h. T-blasts were incubated with CellTrace Yellow Cell Proliferation Kit (#C34567; Thermo Fisher Scientific) diluted 1:500 in PBS supplemented with 2% FBS at 37°C under an atmosphere containing 5% CO 2 for 20 min. Five volumes of prewarmed culture media were added and cells were incubated further for 5 min. ..

    Article Title: Nestin+ Mesenchymal Precursors Generate Distinct Spleen Stromal Cell Subsets and Have Immunomodulatory Function
    Article Snippet: .. Proliferation Assay Isolated CD3+ T cells were stained using a CellTrace Yellow cell proliferation kit (Invitrogen) according to the manufacturer’s instructions, and then were cultured with or without Nes-GFP+ cells treated with PHA (5 μg/ml) for 4 days. ..

    Article Title: Lipid Membrane-Based Antigen Presentation to B Cells Using a Fully Synthetic Ex Vivo Germinal Center Model.
    Article Snippet: Murine recombinant IL-4 (20 ngmL 1, PeptroTech, Rocky Hill, NJ, USA) and BAFF (50 ngmL 1, R&D Systems, Minneapolis, MN, USA) were added in the initial culture medium. .. For fluorescent proliferation analyses, isolated B cells were immediately stained with CellTrace Yellow Cell Proliferation Kit (Invitrogen) according to the manufacturer's instructions prior to culture. .. On day 3 of culture, IL-4 was replaced with recombinant IL-21 (10 ngmL 1, Peptrotech).

    Incubation:

    Article Title: Helper T cell immunity in humans with inherited CD4 deficiency
    Article Snippet: Allogenic Mo were isolated from healthy donor whole blood using EasySep Direct Human PBMC Isolation Kit (#19654; Stemcell Technologies) and Human Pan Monocyte cell isolation kit (#130-096-537; Miltenyi Biotech) in accordance with the manufacturer’s instructions. .. Isolated Mo were incubated in the absence or presence of 5 μg/condition of purified antibodies directed against HLA-ABC (clone G46-2.6, #555551; BD Pharmingen) or HLA-DR, DP, and DQ (clone Tü39, #555557; BD Pharmingen) at 37°C under an atmosphere containing 5% CO 2 for 1 h. T-blasts were incubated with CellTrace Yellow Cell Proliferation Kit (#C34567; Thermo Fisher Scientific) diluted 1:500 in PBS supplemented with 2% FBS at 37°C under an atmosphere containing 5% CO 2 for 20 min. Five volumes of prewarmed culture media were added and cells were incubated further for 5 min. ..

    Purification:

    Article Title: Helper T cell immunity in humans with inherited CD4 deficiency
    Article Snippet: Allogenic Mo were isolated from healthy donor whole blood using EasySep Direct Human PBMC Isolation Kit (#19654; Stemcell Technologies) and Human Pan Monocyte cell isolation kit (#130-096-537; Miltenyi Biotech) in accordance with the manufacturer’s instructions. .. Isolated Mo were incubated in the absence or presence of 5 μg/condition of purified antibodies directed against HLA-ABC (clone G46-2.6, #555551; BD Pharmingen) or HLA-DR, DP, and DQ (clone Tü39, #555557; BD Pharmingen) at 37°C under an atmosphere containing 5% CO 2 for 1 h. T-blasts were incubated with CellTrace Yellow Cell Proliferation Kit (#C34567; Thermo Fisher Scientific) diluted 1:500 in PBS supplemented with 2% FBS at 37°C under an atmosphere containing 5% CO 2 for 20 min. Five volumes of prewarmed culture media were added and cells were incubated further for 5 min. ..

    Proliferation Assay:

    Article Title: Nestin+ Mesenchymal Precursors Generate Distinct Spleen Stromal Cell Subsets and Have Immunomodulatory Function
    Article Snippet: .. Proliferation Assay Isolated CD3+ T cells were stained using a CellTrace Yellow cell proliferation kit (Invitrogen) according to the manufacturer’s instructions, and then were cultured with or without Nes-GFP+ cells treated with PHA (5 μg/ml) for 4 days. ..

    Staining:

    Article Title: Nestin+ Mesenchymal Precursors Generate Distinct Spleen Stromal Cell Subsets and Have Immunomodulatory Function
    Article Snippet: .. Proliferation Assay Isolated CD3+ T cells were stained using a CellTrace Yellow cell proliferation kit (Invitrogen) according to the manufacturer’s instructions, and then were cultured with or without Nes-GFP+ cells treated with PHA (5 μg/ml) for 4 days. ..

    Article Title: Analysis of lipid uptake, storage, and fatty acid oxidation by group 2 innate lymphoid cells
    Article Snippet: Absorbance at 450 nm was measured using an EnspireTM 2300 Multilabel Reader (PerkinElmer). .. Bone marrow ILC2 were stained with CellTrace Yellow Cell Proliferation Kit (Invitrogen, Catalog No. C34573) according to manufacturer’s instructions. ..

    Article Title: Lipid Membrane-Based Antigen Presentation to B Cells Using a Fully Synthetic Ex Vivo Germinal Center Model.
    Article Snippet: Murine recombinant IL-4 (20 ngmL 1, PeptroTech, Rocky Hill, NJ, USA) and BAFF (50 ngmL 1, R&D Systems, Minneapolis, MN, USA) were added in the initial culture medium. .. For fluorescent proliferation analyses, isolated B cells were immediately stained with CellTrace Yellow Cell Proliferation Kit (Invitrogen) according to the manufacturer's instructions prior to culture. .. On day 3 of culture, IL-4 was replaced with recombinant IL-21 (10 ngmL 1, Peptrotech).

    Cell Culture:

    Article Title: Nestin+ Mesenchymal Precursors Generate Distinct Spleen Stromal Cell Subsets and Have Immunomodulatory Function
    Article Snippet: .. Proliferation Assay Isolated CD3+ T cells were stained using a CellTrace Yellow cell proliferation kit (Invitrogen) according to the manufacturer’s instructions, and then were cultured with or without Nes-GFP+ cells treated with PHA (5 μg/ml) for 4 days. ..



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    Thermo Fisher celltrace (yellow cell proliferation kit)
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    Cytokine production and <t>proliferation</t> of murine ILC2 upon treatment with distinct cytokine combinations. Bone marrow-derived (A, B, E, F) or lung-derived (C, D) group 2 innate lymphoid cells (ILC2) were stimulated with either IL-7 only, IL-33 only, or a combination of IL-7 and IL-33 (A, C, E) or IL-2 only, IL-33 only, or a combination of IL-2 and IL-33 (B, D, F) . All cytokines were applied at 10 ng/mL. (A–D) After 24 hours of stimulation, supernatants were harvested and analyzed for IL-5 content by ELISA. (E, F) Proliferation of ILC2 was assessed using <t>CellTrace</t> Yellow Cell Proliferation Kit after 3 days of incubation with respective cytokines. The data representing the IL-33 stimulation is the same for (A–F) . Data are representative of three independent experiments with stimulations performed in duplicates. Data are shown as average ± standard deviation (SD). Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparisons test (p < 0.01 = ** and p < 0.0001 = ****); ND, not detectable.
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    86
    Thermo Fisher celltrace yellow cell proliferation kit invitrogen cat no c34567
    Cytokine production and <t>proliferation</t> of murine ILC2 upon treatment with distinct cytokine combinations. Bone marrow-derived (A, B, E, F) or lung-derived (C, D) group 2 innate lymphoid cells (ILC2) were stimulated with either IL-7 only, IL-33 only, or a combination of IL-7 and IL-33 (A, C, E) or IL-2 only, IL-33 only, or a combination of IL-2 and IL-33 (B, D, F) . All cytokines were applied at 10 ng/mL. (A–D) After 24 hours of stimulation, supernatants were harvested and analyzed for IL-5 content by ELISA. (E, F) Proliferation of ILC2 was assessed using <t>CellTrace</t> Yellow Cell Proliferation Kit after 3 days of incubation with respective cytokines. The data representing the IL-33 stimulation is the same for (A–F) . Data are representative of three independent experiments with stimulations performed in duplicates. Data are shown as average ± standard deviation (SD). Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparisons test (p < 0.01 = ** and p < 0.0001 = ****); ND, not detectable.
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    Cytokine production and proliferation of murine ILC2 upon treatment with distinct cytokine combinations. Bone marrow-derived (A, B, E, F) or lung-derived (C, D) group 2 innate lymphoid cells (ILC2) were stimulated with either IL-7 only, IL-33 only, or a combination of IL-7 and IL-33 (A, C, E) or IL-2 only, IL-33 only, or a combination of IL-2 and IL-33 (B, D, F) . All cytokines were applied at 10 ng/mL. (A–D) After 24 hours of stimulation, supernatants were harvested and analyzed for IL-5 content by ELISA. (E, F) Proliferation of ILC2 was assessed using CellTrace Yellow Cell Proliferation Kit after 3 days of incubation with respective cytokines. The data representing the IL-33 stimulation is the same for (A–F) . Data are representative of three independent experiments with stimulations performed in duplicates. Data are shown as average ± standard deviation (SD). Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparisons test (p < 0.01 = ** and p < 0.0001 = ****); ND, not detectable.

    Journal: Frontiers in Immunology

    Article Title: Analysis of lipid uptake, storage, and fatty acid oxidation by group 2 innate lymphoid cells

    doi: 10.3389/fimmu.2024.1493848

    Figure Lengend Snippet: Cytokine production and proliferation of murine ILC2 upon treatment with distinct cytokine combinations. Bone marrow-derived (A, B, E, F) or lung-derived (C, D) group 2 innate lymphoid cells (ILC2) were stimulated with either IL-7 only, IL-33 only, or a combination of IL-7 and IL-33 (A, C, E) or IL-2 only, IL-33 only, or a combination of IL-2 and IL-33 (B, D, F) . All cytokines were applied at 10 ng/mL. (A–D) After 24 hours of stimulation, supernatants were harvested and analyzed for IL-5 content by ELISA. (E, F) Proliferation of ILC2 was assessed using CellTrace Yellow Cell Proliferation Kit after 3 days of incubation with respective cytokines. The data representing the IL-33 stimulation is the same for (A–F) . Data are representative of three independent experiments with stimulations performed in duplicates. Data are shown as average ± standard deviation (SD). Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparisons test (p < 0.01 = ** and p < 0.0001 = ****); ND, not detectable.

    Article Snippet: Bone marrow ILC2 were stained with CellTrace Yellow Cell Proliferation Kit (Invitrogen, Catalog No. C34573) according to manufacturer’s instructions.

    Techniques: Derivative Assay, Enzyme-linked Immunosorbent Assay, Incubation, Standard Deviation